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Raeghi, Saber
- Immunization of Sheep Against Echinococcus granulosus With Protoscolex Tegumental Surface Antigens
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Materials and Methods: Ten lambs which were infected with CE (positive control), 10 negative control, and 10 test groups of sheep were included in the study. 300 μg emulsion of purified-PSTSA was injected intramuscularly in a two-step immunization on the first and 30 days. Sera were collected immediately before immunization and 6 times with 10-day intervals until 60 days post immunization. Thereafter, the sera were tested for antibodies by indirect hemagglutination test in microtiter plate.
Results: After two immunizations, all the infected animals in test group showed substantial increases in antibody titer. Statistical analysis showed a significant difference between the titer obtained in the test and negative control groups in both phases of immunization (p<0.05).
Conclusion: The results showed that the PSTSA is a promising immunogenic compound for immunization of sheep against CE.
Authors
Manouchehr Valizadeh
1,
Behzad Haghpanah
2,
Alireza Badirzadeh
1,
Elham Roointan
1,
Shirzad Fallahi
3,
Saber Raeghi
4
Affiliations
1 Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR
2 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR
3 Department of Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, IR
4 Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, IR
1 Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR
2 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR
3 Department of Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, IR
4 Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, IR
Source
Veterinary World, Vol 10, No 8 (2017), Pagination: 854-858Abstract
Aim: Cystic echinococcosis (CE) has potential economic effects to both animal products and human health. A vaccine to protect livestock against CE can be effective in reducing economic costs and increasing the livestock products. Protoscolex tegumental surface antigens (PSTSA) used to induce the production of specific antibodies against Echinococcus granulosus in sheep. The tegumental antigens were extracted from viable protoscolices by solubilization in sterile phosphate-buffered saline containing decanoyl-N-methylglucamine.Materials and Methods: Ten lambs which were infected with CE (positive control), 10 negative control, and 10 test groups of sheep were included in the study. 300 μg emulsion of purified-PSTSA was injected intramuscularly in a two-step immunization on the first and 30 days. Sera were collected immediately before immunization and 6 times with 10-day intervals until 60 days post immunization. Thereafter, the sera were tested for antibodies by indirect hemagglutination test in microtiter plate.
Results: After two immunizations, all the infected animals in test group showed substantial increases in antibody titer. Statistical analysis showed a significant difference between the titer obtained in the test and negative control groups in both phases of immunization (p<0.05).
Conclusion: The results showed that the PSTSA is a promising immunogenic compound for immunization of sheep against CE.
Keywords
Cystic Echinococcosis, Echinococcus granulosus, Immunization, Iran, Protoscolex Tegumental Surface Antigens, Sheep.Full Text
- Detection of Species and Molecular Typing of Leishmania in Suspected Patients by Targeting Cytochrome b Gene in Zahedan, Southeast of Iran
Abstract Views :149 |
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Materials and Methods: It was conducted on 145 suspected CL patients in Zahedan city between 2014 and 2016. The smears were initially prepared, air-dried, fixed with absolute methanol, and stained with 10% Giemsa. Then, we examined the stained samples by a light microscope under 1000× magnifications. PCR assay targeted cytochrome b (cyt b) gene using LCBF1 and LCBR2 primers and the products digested by Ssp1 enzymes.
Results: From 145 suspected CL patients, 76 (52.4%) were positive in microscopic examination. In addition, we detected gene of interest (cyt b) in 98 (67.5%). The results of PCR-RFLP indicated that 53/98 (54%) cases were Leishmania major and 45/98 (46%) were Leishmania tropica, and the main species in these areas was L. major.
Conclusion: We concluded that the microscopic examination is not sensitive enough and is not able to distinguish between different Leishmania species. Instead, molecular methods like PCR-RFLP can be appropriately used with promising results.
Authors
Affiliations
1 Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR
2 Department of Parasitology and Mycology, Zahedan University of Medical Sciences, Zahedan, IR
3 Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, IR
4 Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, IR
1 Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR
2 Department of Parasitology and Mycology, Zahedan University of Medical Sciences, Zahedan, IR
3 Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, IR
4 Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, IR
Source
Veterinary World, Vol 11, No 5 (2018), Pagination: 700-705Abstract
Aim: Cutaneous leishmaniasis (CL) is one of the most important health problems that are capable of involving both tropical and subtropical areas, especially in Iran. This cross-sectional study aimed to differentiate the species that are able to cause CL in Zahedan city by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.Materials and Methods: It was conducted on 145 suspected CL patients in Zahedan city between 2014 and 2016. The smears were initially prepared, air-dried, fixed with absolute methanol, and stained with 10% Giemsa. Then, we examined the stained samples by a light microscope under 1000× magnifications. PCR assay targeted cytochrome b (cyt b) gene using LCBF1 and LCBR2 primers and the products digested by Ssp1 enzymes.
Results: From 145 suspected CL patients, 76 (52.4%) were positive in microscopic examination. In addition, we detected gene of interest (cyt b) in 98 (67.5%). The results of PCR-RFLP indicated that 53/98 (54%) cases were Leishmania major and 45/98 (46%) were Leishmania tropica, and the main species in these areas was L. major.
Conclusion: We concluded that the microscopic examination is not sensitive enough and is not able to distinguish between different Leishmania species. Instead, molecular methods like PCR-RFLP can be appropriately used with promising results.
Keywords
Cytochrome b, Leishmania major, Leishmania tropica, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism.Full Text
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